World’s Largest Wildlife Crossing Opens December 2026 on Highway 101

California’s $114 million bridge spans 10-lane Highway 101 in Agoura Hills to save mountain lions from local extinction

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Rex Freiberger Avatar

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Image: Rock Design Associates/National Wildlife Federation

Key Takeaways

  • World’s largest wildlife crossing opens December 2026 spanning 320 by 175 feet
  • $114 million project reconnects fragmented habitats preventing mountain lion local extinction
  • Bridge features 5,000 native plants from 1.1 million hyper-local seeds across 55,925 square feet

Wildlife-vehicle collisions plague Highway 101’s 10 lanes, but the Wallis Annenberg Wildlife Crossing eliminates this deadly barrier starting December 2, 2026. The world’s largest wildlife crossing now spans 320 by 175 feet over one of California’s busiest freeways, reconnecting fragmented habitats that have isolated mountain lions and other species for over three decades.

Engineering a Living Bridge

Since Earth Day 2022, crews have installed 82 large concrete beams, creating 55,925 square feet of vegetated overpass. The $114 million project sits 60% complete in Agoura Hills, northwest of Los Angeles, where the Santa Monica Mountains meet urban sprawl.

Mountain lions face local extinction without genetic diversity—this crossing provides their lifeline to populations in the Simi Hills and beyond. Workers are now spreading 3 million cubic feet of soil and planting 5,000 native plants grown from 1.1 million hyper-local seeds. Exclusionary fencing guides animals toward the crossing while berms block highway noise and light pollution.

Conservation Meets Education

The crossing targets species from bobcats to black bears, all struggling with habitat fragmentation in Southern California’s urban-wilderness interface.

A public viewing platform allows visitors to observe without disturbing wildlife movement, supporting California’s ambitious 30×30 conservation initiative. The site honors its location on Chumash homelands while serving as a living laboratory for similar projects nationwide.

Governor Newsom’s Earth Day announcement marked a milestone for this first California highway bridge designed exclusively for wildlife passage—visible proof that conservation and infrastructure can coexist on one of America’s most congested corridors.

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